NASA Spaceline Current Awareness 9 November 2012 (Recent Space Life Science Research Results)

Next list: November 23 or 30, 2012. Papers deriving from NASA support:

1
Crucian B, Stowe R, Mehta S, Uchakin P, Quiriarte H, Pierson D, Sams C.
Immune system dysregulation cccurs during short duration spaceflight on board the Space Shuttle.
J Clin Immunol. 2012 Oct 26. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23100144
(PIs: R. Stowe, D. Pierson, C. Sams)
NOTE: Shuttle results (nine missions). From Discussion: “It is therefore possible that during spaceflight, a condition of unimpaired/enhanced innate immune response exists, but with dysregulation of adaptive immune cell distribution and function.”

2
Mehta SK, Crucian BE, Stowe RP, Simpson RJ, Ott CM, Sams CF, Pierson DL.
Reactivation of latent viruses is associated with increased plasma cytokines in astronauts.
Cytokine. 2012 Oct 26. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23107825
(PI: D.L. Pierson)
NOTE: Shuttle results (eight missions).

3
Martin DS, Caine TL, Matz T, Lee SM, Stenger MB, Sargsyan AE, Platts SH.
Virtual guidance as a tool to obtain diagnostic ultrasound for spaceflight and remote environments.
Aviat Space Environ Med. 2012 Oct;83(10):995-1000.
http://www.ncbi.nlm.nih.gov/pubmed/23066623

4
Asadi AK, Cronin MV, Sa RC, Theilmann RJ, Holverda S, Hopkins SR, Buxton RB, Prisk GK.
Spatial-temporal dynamics of pulmonary blood flow in the healthy human lung in response to altered FIO2.
J Appl Physiol. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23104691
(PIs: R.C. Sa, NSBRI Postdoctoral Fellowship Program; G.K. Prisk)

5
Zhao L, Tanjung N, Swarnkar G, Ledet E, Yokota H.
Regulation of eIF2 phosphorylation in hindlimb-unloaded and STS-135 space-flown mice.
Adv Space Res. 2012 Sep 1;50(5):576-83.
http://dx.doi.org/10.1016/j.asr.2012.05.024
(PI: H. Yokota)
NOTE: STS-135 results.

6
Spatz JM, Ellman R, Cloutier AM, Louis L, van Vliet M, Suva LJ, Dwyer D, Stolina M, Ke HZ, Bouxsein ML.
Sclerostin antibody inhibits skeletal deterioration due to reduced mechanical loading.
J Bone Miner Res. 2012 Oct 29. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23109229
(PIs: M.L. Bouxsein; NSBRI?; R. Ellman, NASA Harriet G. Jenkins Predoctoral Fellowship)

7
Liu H, Blough ER, Arvapalli R, Wang Y, Reiser PJ, Paturi S, Katta A, Harris R, Nepal N, Wu M.
Regulation of contractile proteins and protein translational signaling in disused muscle.
Cell Physiol Biochem. 2012 Oct 15;30(5):1202-14. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23075566
NOTE: This article may be obtained online without charge.

8
Shukitt-Hale B, Lau FC, Cheng V, Luskin K, Carey AN, Carrihill-Knoll K, Rabin BM, Joseph JA.
Changes in gene expression in the rat hippocampus following exposure to 56/Fe particles and protection by berry diets.
Cent Nerv Syst Agents Med Chem. 2012 Oct 17. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23092409
(PI: B.M. Rabin)

9
Romero-Weaver AL, Kennedy AR.
Comparison of two methods for the determination of the effects of ionizing radiation on blood cell counts in mice.
Int J Biomed Sci. 2012 Mar 15;8(1):7-15.
http://ijbs.org/User/All-by-date.aspx
(PI: A.R. Kennedy/ Center of Acute Radiation Research)
NOTE: This article may be obtained online without charge.

10
Rueger M, St. Hilaire MA, Brainard G, Khalsa SB, Kronauer RE, Czeisler CA, Lockley SW.
Human phase response curve to a single 6.5-h pulse of short-wavelength light.
J Physiol. 2012 Oct 22. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23090946
(PIs: G. Brainard, C.A. Czeisler, S.W. Lockley)

11
Giesy TJ, Wang Y, LeVan MD.
Measurement of mass transfer rates in adsorbents: New combined-technique frequency response apparatus and application to CO2 in 13X zeolite.
Ind Eng Chem Res. 2012 Sep 5;51(35):11509-17.
http://dx.doi.org/10.1021/ie3014204
(PI: J.A. Ritter)

12
Vaishampayan P, Probst AJ, La Duc MT, Bargoma E, Benardini JN, Andersen GL, Venkateswaran K.
New perspectives on viable microbial communities in low-biomass cleanroom environments.
ISME J. 2012 Oct 11. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23051695

Other papers of interest:

1
Blue RS, Riccitello JM, Tizard J, Hamilton RJ, Vanderploeg JM.
Commercial spaceflight participant G-force tolerance during centrifuge-simulated suborbital flight.
Aviat Space Environ Med. 2012 Oct;83(10):929-34.
http://www.ncbi.nlm.nih.gov/pubmed/23066613
NOTE: From Methods: “This was a retrospective observational study of the subjective and objective parameters recorded during the physiologic training of the initial group of Virgin Galactic future suborbital spaceflight participants at the National Aerospace Training and Research (NASTAR) Center centrifuge (Environmental Tectonics Corp., Southampton, PA).”

2
Kim DH, Parsa CF.
Space flight and disc edema.
Ophthalmology. 2012 Nov;119(11):2420-1.
http://www.ncbi.nlm.nih.gov/pubmed/23122477
NOTE: Correspondence.

3
Heydari M, Boutcher YN, Boutcher SH.
High-intensity intermittent exercise and cardiovascular and autonomic function.
Clin Auton Res. 2012 Oct 29. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23104677

4
Bozhokin SV, Lesova EM, Samoilov VO, Tolkachev PI.
Wavelet analysis of nonstationary heart rate variability in a head-up tilt-table test.
Biophysics. 2012 Jul;57(4):530-43.
http://dx.doi.org/10.1134/S0006350912040033
NOTE: This paper is a translation in English of the Russian original published in Biofizika. 2012 Jul-Aug;57(4):696-712: http://www.ncbi.nlm.nih.gov/pubmed/23035538

5
Item F, Nocito A, Thoeny S, Baechler T, Boutellier U, Wenger RH, Toigo M.
Combined whole-body vibration, resistance exercise, and sustained vascular occlusion increases PGC-1 and VEGF mRNA abundances.
Eur J Appl Physiol. 2012 Oct 20. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23086295
NOTE: This article may temporarily be obtained online without charge: http://dx.doi.org/10.1007/s00421-012-2524-4

6
Albi E, Curcio F, Spelat R, Lazzarini A, Lazzarini R, Loreti E, Ferri I, Ambesi-Impiombato FS.
Observing the mouse thyroid sphingomyelin under space conditions: A case study from the MDS mission in comparison with hypergravity conditions.
Astrobiology. 2012 Nov;12(11):1035-41. Epub 2012 Oct 19.
http://www.ncbi.nlm.nih.gov/pubmed/23082746
NOTE: ISS results.

7
Aaron M, Wright S, Gooch J, Harvey R, Davis R, Reilly C.
Stability of laser-assisted in situ keratomileusis (LASIK) at altitude.
Aviat Space Environ Med. 2012 Oct;83(10):958-61.
http://www.ncbi.nlm.nih.gov/pubmed/23066617

8
Mekjavic IB, Debevec T, Amon M, Keramidas ME, Kounalakis SN.
Intermittent normobaric hypoxic exposures at rest: Effects on performance in normoxia and hypoxia.
Aviat Space Environ Med. 2012 Oct;83(10):942-50.
http://www.ncbi.nlm.nih.gov/pubmed/23066615

9
Schellart NA, van Rees Vellinga TP, van Dijk FJ, Sterk W.
Doppler bubble grades after diving and relevance of body fat.
Aviat Space Environ Med. 2012 Oct;83(10):951-7.
http://www.ncbi.nlm.nih.gov/pubmed/23066616

10
Petrassi FA, Hodkinson PD, Walters PL, Gaydos SJ.
Hypoxic hypoxia at moderate altitudes: Review of the state of the science.
Aviat Space Environ Med. 2012 Oct;83(10):975-84.
http://www.ncbi.nlm.nih.gov/pubmed/23066620

11
Li X, Ding L, Hedge A, Hu H, Qin Z, Zhou Q.
An experimental study on the ergonomics indices of partial pressure suits.
Appl Ergon. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23102522

12
Bassett-Gunter RL, Martin Ginis KA, Latimer-Cheung AE.
Do you want the good news or the bad news? Gain-versus loss-framed messages following health risk information: The effects on leisure time physical activity beliefs and cognitions.
Health Psychol. 2012 Oct 22. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23088175
NOTE: The stick works better than the carrot.

13
Maki T, Yamamoto D, Nakanishi S, Iida K, Iguchi G, Takahashi Y, Kaji H, Chihara K, Okimura Y.
Branched-chain amino acids reduce hindlimb suspension-induced muscle atrophy and protein levels of atrogin-1 and MuRF1 in rats.
Nutr Res. 2012 Sep;32(9):676-83. Epub 2012 Sep 17.
http://www.ncbi.nlm.nih.gov/pubmed/23084640

14
Kim JH, Thompson LV.
Inactivity, age, and exercise: Single muscle fiber power generation.
J Appl Physiol. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23104693

15
Kanazashi M, Okumura Y, Al-Nassan S, Murakami S, Kondo H, Nagatomo F, Fujita N, Ishihara A, Roy RR, Fujino H.
Protective effects of astaxanthin on capillary regression in atrophied soleus muscle of rats.
Acta Physiol (Oxf). 2012 Oct 22. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23088455

16
Lundberg TR, Fernandez-Gonzalo R, Gustafsson T, Tesch PA.
Aerobic exercise does not compromise muscle hypertrophy response to short-term resistance training.
J Appl Physiol. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23104700

17
Nagpal P, Plant PJ, Correa J, Bain A, Takeda M, Kawabe H, Rotin D, Bain JR, Batt JA.
The ubiquitin ligase Nedd4-1 participates in denervation-induced skeletal muscle atrophy in mice.
PLoS One. 2012;7(10):e46427. Epub 2012 Oct 26.
http://www.ncbi.nlm.nih.gov/pubmed/23110050
NOTE: This article may be obtained online without charge.

18
Ponomareva EV, Ogneva IV.
Contractile properties, transversal stiffness and cytoskeletal protein content in Mongolian gerbils soleus fibers under long-term hindlimb suspension.
Biophysics. 2012 Jul;57(4):519-24.
http://dx.doi.org/10.1134/S0006350912040148
NOTE: This paper is a translation in English of the Russian original published in Biofizika. 2012 Jul-Aug;57(4):683-9: http://www.ncbi.nlm.nih.gov/pubmed/23035536

19
Llano-Diez M, Renaud G, Andersson M, Gonzales Marrero H, Cacciani N, Engquist H, Corpeno R, Artemenko K, Bergquist J, Larsson L.
Mechanisms underlying intensive care unit muscle wasting and effects of passive mechanical loading.
Crit Care. 2012 Oct 26;16:R209. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23098317
NOTE: This article may be obtained online without charge.

20
Schlecht SH, Pinto DC, Agnew AM, Stout SD.
Brief communication: The effects of disuse on the mechanical properties of bone: What unloading tells us about the adaptive nature of skeletal tissue.
Am J Phys Anthropol. 2012 Oct 19. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23086658.

21
Klein-Nulend J, Bakker AD, Bacabac RG, Vatsa A, Weinbaum S.
Mechanosensation and transduction in osteocytes.
Bone. 2012 Oct 18. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23085083

22
Mo C, Romero-Suarez S, Bonewald L, Johnson M, Brotto M.
Prostaglandin E2: From clinical applications to its potential role in bone-muscle crosstalk and myogenic differentiation.
Recent Pat Biotechnol. 2012 Oct 22. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23092433

23
Zhu B, Guo H, Hao XJ, Fu Q, Hu SM.
[Mechanism of weightlessness osteoporosis and preventive and therapeutic effect of traditional Chinese medicine]
Zhongguo Gu Shang. 2012 Jul;25(7):611-6. Chinese.
http://www.ncbi.nlm.nih.gov/pubmed/23116001
NOTE: Translated title of journal is China Journal of Orthopaedics and Traumatology.

24
Sengul A, Subburaman M, Kesavan C.
Bone response to loading in mice with targeted disruption of the cartilage oligomeric matrix protein gene.
Physiol Res. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23098652
NOTE: This article may be obtained online without charge.

25
Kleiman NJ.
Radiation cataract.
Ann ICRP. 2012 Oct;41(3-4):80-97. Epub 2012 Aug 22.
http://www.ncbi.nlm.nih.gov/pubmed/23089007

26
Menzel HG, Harrison J.
Effective dose: a radiation protection quantity.
Ann ICRP. 2012 Oct;41(3-4):117-23. Epub 2012 Aug 22.
http://www.ncbi.nlm.nih.gov/pubmed/23089010
NOTE: This issue has a number of articles on the topics of radiation, dose, and risk: http://www.sciencedirect.com/science/journal/01466453/41/3

27
Endo A, Sato T.
Radiation transport calculations for cosmic radiation.
Ann ICRP. 2012 Oct;41(3-4):142-53. Epub 2012 Aug 22.
http://www.ncbi.nlm.nih.gov/pubmed/23089013

28
Deperas-Standylo J, Lee R, Nasonova E, Ritter S, Gudowska-Nowak E.
Production and distribution of aberrations in resting or cycling human lymphocytes following Fe-ion or Cr-ion irradiation: Emphasis on single track effects.
Adv Space Res. 2012 Sep 1;50(5):584-97.
http://dx.doi.org/10.1016/j.asr.2012.05.007

29
Wang B, Ninomiya Y, Tanaka K, Maruyama K, Vares G, Eguchi-Kasai K, Nenoi M.
Adaptive response of low linear energy transfer X-rays for protection against high linear energy transfer accelerated heavy ion-induced teratogenesis.
Birth Defects Res B Dev Reprod Toxicol. 2012 Oct 26. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23109298

30
Obara T.
Space environment data acquisition with Kibo exposed facility on the International Space Station (ISS).
Electronics Commun Japan. 2012 Sep;95(9):10-6.
http://dx.doi.org/10.1002/ecj.11418
NOTE: About equipment on ISS.

31
Liang X, Zhang L, Wan Y, Yu X, Guo Y, Chen X, Tan C, Huang T, Shen H, Chen X, Li H, Lv K, Sun F, Chen S, Guo J.
Changes in the diurnal rhythms during a 45-day head-down bed rest.
PLoS One. 2012;7(10):e47984. Epub 2012 Oct 24.
http://www.ncbi.nlm.nih.gov/pubmed/23110150
NOTE: Bed rest study. This article may be obtained online without charge.

32
Borghini G, Astolfi L, Vecchiato G, Mattia D, Babiloni F.
Measuring neurophysiological signals in aircraft pilots and car drivers for the assessment of mental workload, fatigue and drowsiness.
Neurosci Biobehav Rev. 2012 Oct 30. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23116991

33
Allison RS, Zacher JE, Kirollos R, Guterman PS, Palmisano S.
Perception of smooth and perturbed vection in short-duration microgravity.
Exp Brain Res. 2012 Dec;223(4):479-87. Epub 2012 Oct 2.
http://www.ncbi.nlm.nih.gov/pubmed/23111427
NOTE: Parabolic flight results. This article may temporarily be obtained online without charge.

34
Baier B, Thoemke F, Wilting J, Heinze C, Geber C, Dieterich M.
A pathway in the brainstem for roll-tilt of the subjective visual vertical: Evidence from a lesion-behavior mapping study.
J Neurosci. 2012 Oct 24;32(43):14854-8.
http://www.ncbi.nlm.nih.gov/pubmed/23100408

35
Ferre ER, Vagnoni E, Haggard P.
Galvanic vestibular stimulation influences randomness of number generation.
Exp Brain Res. 2012 Oct 31. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23111396
NOTE: This article may temporarily be obtained online without charge.

36
Palmer CJ, Riccio GE, Van Emmerik RE.
Orienting under load: Intrinsic dynamics and postural affordances for visual perception.
Ecol Psychol. 2012;24(2):95-121.
http://dx.doi.org/10.1080/10407413.2012.673970

37
Tsutsumi T, Ikeda T, Fukuoka Y, Watanabe K, Kikuchi S.
Optokinetic stimulation can break Listing’s law without induction of eye movement.
Auris Nasus Larynx. 2012 Oct 24. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23103150

38
Kang H, Fan Y, Sun A, Jia X, Deng X.
Simulated microgravity exposure modulates the phenotype of cultured vascular smooth muscle cells.
Cell Biochem Biophys. 2012 Oct 25. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23097024
NOTE: This article may temporarily be obtained online without charge.

39
Hao T, Li JJ, DU ZY, Duan CM, Wang YM, Wang CY, Song JP, Wang LJ, Li YH, Wang Y.
[Cordyceps sinensis enhances lymphocyte proliferation and CD markers expression in simulated microgravity environment]
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Oct;20(5):1212-5. Chinese.
http://www.ncbi.nlm.nih.gov/pubmed/23114150
NOTE: Translated title of journal is Chinese Association of Pathophysiology.

40
Blasi P, Luca G, Mancuso F, Schoubben A, Calvitti M, Giovagnoli S, Basta G, Becchetti E, Ricci M, Calafiore R.
Conformal polymer coatings for pancreatic islets transplantation.
Int J Pharm. 2012 Oct 16. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23078858
NOTE: This research used simulated microgravity, obtained with a rotating cell culture system bioreactor.

41
Ladoux B, Nicolas A.
Physically based principles of cell adhesion mechanosensitivity in tissues.
Rep Prog Phys. 2012 Nov;75(11):116601. Epub 2012 Oct 19.
http://www.ncbi.nlm.nih.gov/pubmed/23085962
NOTE: This article may be obtained online without charge: http://iopscience.iop.org/0034-4885/75/11/116601

42
Maksaev G, Haswell ES.
MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions.
Proc Natl Acad Sci U S A. 2012 Oct 29. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23112188

43
Dong YY, Gao WY, Zhang JZ, Zuo BM, Huang LQ.
Quantification of four active ingredients and fingerprint analysis of Licorice (Glycyrrhiza uralensis Fisch.) after spaceflight by HPLC-DAD.
Res Chem Intermed. 2012 Oct;38(8):1719-31.
http://dx.doi.org/10.1007/s11164-012-0497-x
NOTE: Results from plants grown from seeds flown on a Chinese satellite (Shenzhou).

44
Hashiguchi Y, Tasaka M, Morita MT.
Mechanism of higher plant gravity sensing.
Am J Bot. 2012 Oct 31. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23115136
NOTE: This article may temporarily be obtained online without charge.

45
Clore AM.
Cereal grass pulvini: Agronomically significant models for studying gravitropism signaling and tissue polarity.
Am J Bot. 2012 Nov 2. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/23125431
NOTE: This article may temporarily be obtained online without charge.

46
Swarup R, Peret B.
AUX/LAX family of auxin influx carriers–an overview.
Front Plant Sci. 2012;3:225. Epub 2012 Oct 18.
http://www.ncbi.nlm.nih.gov/pubmed/23087694
NOTE: This article may be obtained online without charge.

47
dos Santos MA, Fachel FN, Nava MJ, Astarita LV, Collin P, Russomano T.
Effect of hypergravity simulation on carrot germination and growth.
Aviat Space Environ Med. 2012 Oct;83(10):1011-2.
http://www.ncbi.nlm.nih.gov/pubmed/23066627
NOTE: Author affiliation: The Joan Vernikos Aerospace Pharmacy Lab and the Microgravity Centre, PUCRS, Brazil. This article may be obtained online without charge: http://www.ingentaconnect.com/content/asma/asem/2012/00000083/00000010/a…

48
Atiashkin DA, Bykov EG, Ilyin EA, Pashkov AN.
[Jejunum intersticium in Mongolian gerbils after the flight on spacecraft Foton-M3]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):8-13. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074944
NOTE: Foton M3 results.

49
Gulimova VI, Barabanov VM, Berdiev RK, Okshtein IL, Rakov DV, Landau IN, Saveliev SV.
[Supporting life of Pachydactylus turneri in a projected experiment aboard robotic spacecraft: Choosing feed]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):14-9. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074945

50
Tatarkin SV, Shafirkin AV, Mukhamedieva LN, Barantseva MYu, Ivanova SM.
[Adaptation processes in mice during chronic combined exposure to radiation and chemical compounds (acetone, ethanol, acetaldehyde) innate to exploration missions]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):20-7. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074946

51
Piruzian LA, Protasova OV, Maksimova IA, Morukov BV, Protasov SV, Ushakov IB.
[Magnesium level in human organism during 105-day isolation]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):28-31. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074947

52
Krasnov IB, Diachkova LN.
[Change in formation of the somatosensory cortex ultrastructure in rats due to hypergravity in pre- and postnatal ontogenesis]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):32-5. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074948

53
Nikolaev VP.
[Simulation of tissue nitrogen supersaturation and the risk of tissue lesion by gas bubbles at non-stop dives]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):45-51. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074951

54
Alchinova IB, Khlebnikova NN, Karganov MYu.
[Information capability of the test with radioadaptive response in functional evaluation of pilots]
Aviakosm Ekolog Med. 2012 May-Jun;46(3):56-63. Russian.
http://www.ncbi.nlm.nih.gov/pubmed/23074953