Space Stations

NASA Spaceline Current Awareness List #1,010 29 July 2022 (Space Life Science Research Results)

By Keith Cowing
Press Release
July 29, 2022
Filed under , , , ,
NASA Spaceline Current Awareness List #1,010 29 July 2022 (Space Life Science Research Results)
Astronaut Samantha Cristoforetti participates in the Acoustic Diagnostics study (July 8, 2022)

Call for articles to cite in the weekly lists: Authors at NASA Centers and NASA PIs—do you have an article that has recently published or will publish in the upcoming weeks within a peer-reviewed journal and is in the scope of space life sciences? If so, send it our way! Send your article to the email address mentioned above. Articles received by Wednesday will appear within that week’s list—articles received after Wednesday will appear the following week.

Papers deriving from NASA support:


Jasien JV, Laurie SS, Lee SMC, Martin DS, Kemp DT, Ebert DJ, Ploutz-Snyder RJ, Marshall-Goebel K, Alferova IV, Sargsyan AE, Danielson RW, Hargens AR, Dulchavsky SA, Stenger MB, Macias BR.

Noninvasive indicators of intracranial pressure before, during, and after long-duration spaceflight.

J Appl Physiol (1985). 2022 Jul 21. Online ahead of print.

PIs: A.R. Hargens, S.A. Dulchavsky

Note: From the abstract: “Weightlessness induces a cephalad shift of blood and cerebrospinal fluid that may increase intracranial pressure (ICP) during spaceflight, while lower body negative pressure (LBNP) may provide an opportunity to caudally redistribute fluids and lower ICP. To investigate the effects of spaceflight and LBNP on noninvasive indicators of ICP (nICP), we studied thirteen crewmembers before and after spaceflight in seated, supine, and 15° head-down tilt postures, and at ~45 and ~150 days of spaceflight with and without 25 mmHg LBNP. We used 4 techniques to quantify nICP: cerebral and cochlear fluid pressure (CCFP), otoacoustic emissions (OAE), ultrasound measures of optic nerve sheath diameter (ONSD), and ultrasound-based internal jugular vein pressure (IJVp).”

Journal Impact Factor: 3.880

Funding: “NNJ11ZSA002NA/National Aeronautics and Space Administration (NASA); NNX13AJ12G/National Aeronautics and Space Administration (NASA); NNX13AK30G/National Aeronautics and Space Administration (NASA).”


Mylabathula PL, Diak DM, Baker FL, Niemiro GM, Markofski MM, Crucian BE, Katsanis E, Simpson RJ.

IL-2 and zoledronic acid therapy restores the in vivo anti-leukemic activity of human lymphocytes pre-exposed to simulated microgravity.

Front Biosci (Landmark Ed). 2022 Jul 8;27(7):215. Review.

PI: R.J. Simpson

Note: A rotary cell culture system was used in this study. This article may be obtained online without charge.

Journal Impact Factor: 4.009

Funding: “These studies were supported by NASA grants NNX16AB29G to RJS and 80NSSC19K1059 to RJS, EK and PLM.”


Cekanaviciute E, Tran D, Nguyen H, Lopez Macha A, Pariset E, Langley S, Babbi G, Malkani S, Penninckx S, Schisler JC, Nguyen T, Karpen GH, Costes SV.

Mouse genomic associations with in vitro sensitivity to simulated space radiation.

Life Sci Space Res. 2022 Jul 28. Online ahead of print.

PI: S.V. Costes

Note: This article may be obtained online without charge.

Journal Impact Factor: 2.730

Funding: “This work was supported by National Aeronautics and Space Administration NNJ16HP24I to S.V.C. (Principal Investigator – PI) and by the Low Dose Scientific Focus Area, Office of Biological and Environmental Research, U.S. Department of Energy under Contract No. DE AC02-05CH11231 to G. H. Karpen (PI) and S.V.C. (Co-PI).”


Britten RA, Fesshaye A, Tidmore A, Blackwell AA.

Similar loss of executive function performance after exposure to low (10 cGy) doses of single (4

He) ions and the multi-ion GCRSim beam.

Radiat Res. 2022 Jul 20. Online ahead of print.

PI: R.A. Britten

Note: From the abstract: “While astronauts are trained to deal with multiple issues that they are likely to encounter during a mission, it is likely that some problems will arise that astronauts have no direct experience in resolving. During International Space Station (ISS) missions, astronauts can rely on Mission Control to help resolve complex problems, however during the long-duration space missions planned to the Moon and Mars, astronauts will have to act more autonomously, thus the ability of astronauts to conduct executive function will be critical for problem solving during deep space missions. Several studies have shown that exposure to space radiation results in decreased executive function performance. However, to date these studies have used single ions; whereas, there is a complex mixture of ion species and energies within the space-radiation spectrum that astronauts will be exposed to. Thus, there is some concern that the neurocognitive impairments reported from single ion studies will not be representative of the severity, frequency or nature of cognitive deficits that arise following exposure to more complex space-radiation spectra. The current study has determined the relative impact that isodoses of He ions or the simplified 6-ion-galactic cosmic ray simulation (GCRSim) beams had on the performance of male Wistar rats in executive function tasks, attentional set shifting (ATSET) task and unconstrained cognitive flexibility (UCFlex).”

Journal Impact Factor: 2.841

Funding: “This work was funded by NASA grant support NNX14AE73G (RAB).”


Ma N, Chen D, Lee JH, Kuri P, Hernandez EB, Kocan J, Mahmood H, Tichy ED, Rompolas P, Mourkioti F.

Piezo1 regulates the regenerative capacity of skeletal muscles via orchestration of stem cell morphological states.

Sci Adv. 2022 Mar 18;8(11):eabn0485.

PI: F. Mourkioti

Note: From the abstract: “Muscle stem cells (MuSCs) are essential for tissue homeostasis and regeneration, but the potential contribution of MuSC morphology to in vivo function remains unknown. Here, we demonstrate that quiescent MuSCs are morphologically heterogeneous and exhibit different patterns of cellular protrusions.”

Journal Impact Factor: 14.957

Funding: “This study was supported by startup funds from Perelman School of Medicine, the NASA (80NSSC19K1602), and the NIH/National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01HL146662) to F.M. Two-photon microscopy was supported by the NIH (S10 OD021633-01).”


Letourneau J, Holmes ZC, Dallow EP, Durand HK, Jiang S, Carrion VM, Gupta SK, Mincey AC, Muehlbauer MJ, Bain JR, David LA.

Ecological memory of prior nutrient exposure in the human gut microbiome.

ISME J. 2022 Jul 23.

PI: L.A. David

Note: From the abstract: “Many ecosystems have been shown to retain a memory of past conditions, which in turn affects how they respond to future stimuli. In microbial ecosystems, community disturbance has been associated with lasting impacts on microbiome structure. However, whether microbial communities alter their response to repeated stimulus remains incompletely understood. Using the human gut microbiome as a model, we show that bacterial communities retain an ‘ecological memory’ of past carbohydrate exposures.”

Journal Impact Factor: 11.217

Funding: “This work was supported by National Institutes of Health grant 1R01DK116187, Office of Naval Research grant N00014-18-1-2616, Translational Research Institute through Cooperative Agreement NNX16AO69A, the Damon Runyon Cancer Research Foundation, and the UNC CGIBD (NIDDK P30DK034987). This study used a high-performance computing facility partially supported by grant 2016-IDG-1013 (HARDAC+: Reproducible HPC for Next-Generation Genomics) from the North Carolina Biotechnology Center. JRB received salary support from NIH 5P30DK124723, 5R01DK117491, 1U24DK129557, and 2P30AG027816.”


Johnson DA, Czeisler CA.

Chapter 1 – Components of normal human sleep.

In: Nieto FJ, Petersen DJ, eds. Foundations of Sleep Health. London: Academic Press, 2022. p. 1-12.

PI: C.A. Czeisler

Funding: “Dr. Johnson is supported in part by the National Heart, Lung, and Blood Insitute K01HL138211. Dr. Czeisler is supported in part by: grants from the National Institute of Occupational Safety and Health (R01-OH-011773), the National Health, Lung, and Blood Institute (R01-HL-148704, R01-HL-132556, R01-HL-103607 and U01-HL-111478), the National Institute on Aging (P01-AG-009975), and the National Aeronautics and Space Administration (NNX15AC14G); and a contract from the Federal Avaiation Administration (6973GH-19-D-00066 from solicitation 6973GH-19-R-00147).”


Boutros SW, Kessler K, Unni VK, Raber J.

Infusion of etoposide in the CA1 disrupts hippocampal immediate early gene expression and hippocampus-dependent learning.

Sci Rep. 2022 Jul 27;12:12834.

PI: J. Raber/NSCOR

Journal Impact Factor: 4.996

Funding: “This work was partially supported by a Tartar Trust Fellowship, an OHSU SOM Pilot Fund, an NSF Graduate Research Fellowship award, NASA NSCOR G-00066-4, R21 CA223461, R21 AG065914, NS102227, F31 AG067629, Knight CVP-003, and the development accounts of Drs. Unni and Raber.”


Other papers of interest:


Thamer S, Buckey JC Jr

First void urinary calcium for tracking bone loss and kidney stone risk in space.

Aerosp Med Hum Perform. 2022 Jul;93(7):546-50.

Note: From the abstract: “For 8 weeks, three men and three women collected void-by-void 24-h urine samples weekly. Uca [urinary calcium] concentration was analyzed using a calcein-based system. Uca concentrations were ranked among all samples from each person. FMV [first morning void] and non-FMV (nFMV) Uca concentrations were compared with a Mann Whitney U-test. The probability that an FMV would capture the highest Uca concentration in a day was assessed.”


Schoenmaekers C, De Laet C, Kornilova L, Glukhikh D, Moore S, MacDougall H, Naumov I, Fransen E, Wille L, Jillings S, Wuyts FL.

Ocular counter-roll is less affected in experienced versus novice space crew after long-duration spaceflight.

npj Microgravity. 2022 Jul 20;8:27.

Note: ISS results. This article may be obtained online without charge.


Sommariva S, Romoli G, Vallarino E, di Fino L, Sorrentino A, Amantini GS, Sannita WG, Piana M, Narici L.

EEG in extreme conditions: An advanced analysis pipeline for the human electroencephalographic signals recorded in space during the ALTEA experiment.

Life Sci Space Res. 2022 Jul 29. Online ahead of print.

Note: From the abstract: “The Anomalous Long Term Effects in Astronauts (ALTEA) project originally aimed at disentangling the mechanisms behind astronauts’ perception of light flashes. To this end, an experimental apparatus was set up in order to concurrently measure the tracks of cosmic radiation particles in the astronauts’ head and the electroencephalographic (EEG) signals generated by their brain. So far, the ALTEA data set has never been analyzed with the broader intent to study possible interference between cosmic radiation and the brain, regardless of light flashes. The aim of this work is to define a pipeline to systematically pre-process the ALTEA EEG data.”


Mian A, Aamir Mian M.

Space medicine: Inspiring a new generation of physicians.

Postgrad Med J. 2022 Jul 20;postgradmedj-2022-141875. Online ahead of print.


Putt KS, Du Y, Fu H, Zhang Z-Y.

High-throughput screening strategies for space-based radiation countermeasure discovery.

Life Sci Space Res. 2022 Jul 21. Online ahead of print.

Note: This article may be obtained online without charge.


Ramalho TP, Chopin G, Pérez-Carrascal OM, Tromas N, Verseux C.

Selection of Anabaena sp. PCC 7938 as a cyanobacterium model for biological ISRU on Mars.

Appl Environ Microbiol. 2022 Jul 12;e0059422. Online ahead of print.

Note: From the abstract: “Crewed missions to Mars are expected to take place in the coming decades. After short-term stays, a permanent presence will be desirable to enable a wealth of scientific discoveries. This will require providing crews with life-support consumables in amounts that are too large to be imported from Earth. Part of these consumables could be produced on site with bioprocesses, but the feedstock should not have to be imported. A solution under consideration lies in using diazotrophic, rock-weathering cyanobacteria as primary producers: fed with materials naturally available on site, they would provide the nutrients required by other organisms. This concept has recently gained momentum but progress is slowed by a lack of consistency across contributing teams, and notably of a shared model organism. With the hope to address this issue, we present the work performed to select our current model.”


Saehle T.

Cerebral hemodynamics during exposure to hypergravity (+Gz

) or microgravity (0 G).

Aerosp Med Hum Perform. 2022 Jul;93(7):581-92.

Note: From the abstract: “A systematic PEO (Population, Exposure, Outcome) search was done in PubMed and Web of Science, addressing studies on how elevated +Gz

 forces or absence of such may impact cerebral hemodynamics. All primary research containing anatomical or physiological data on relevant intracranial parameters were included. Quality of the evidence was analyzed using the GRADE tool.”


May JM, Shankavaram U, Bylicky MA, Chopra S, Scott K, Martello S, Thrall K, Axtelle J, Menon N, Coleman CN, Aryankalayil MJ.

Serum RNA biomarkers for predicting survival in non-human primates following thoracic radiation.

Sci Rep. 2022 Jul 19;12:12333.

Note: This article may be obtained online without charge.


Baranowski RW, Braun JL, Vandenboom R, Fajardo VA.

Neurogranin inhibits calcineurin in murine soleus muscle: Effects of heterozygous knockdown on muscle adaptations to tenotomy and fatigue resistance.

Biochem Biophys Res Commun. 2022 Oct 1;623:89-95.

Note: Hindlimb unloading results.


Hurley KL, Bassett JR, Monroy JA.

Active muscle stiffness is reduced during rapid unloading in muscles from TtnD112-158 mice with a large deletion to PEVK titin.

J Exp Biol. 2022 Jul 25. Online ahead of print.

Note: From the abstract: “Evidence suggests that the giant muscle protein, titin functions as a tunable spring in active muscle. However, the mechanisms for increasing titin stiffness with activation are not well understood. Previous studies have suggested that during muscle activation, titin binds to actin, which engages the PEVK [Proline, Glutamate, Valine and Lysine-rich] region of titin thereby increasing titin stiffness. In this study, we investigated the role of PEVK titin in active muscle stiffness during rapid unloading.”


Safa BN, Bahrani Fard MR, Ethier CR.

In vivo biomechanical assessment of iridial deformations and muscle contractions in human eyes.

J R Soc Interface. 2022 Jul 6;19(192):20220108.

Note: This article may be obtained online without charge.


Wang R, Shan H, Zhang G, Li Q, Wang J, Yan Q, Li E, Diao Y, Wei L.

An inulin-type fructan (AMP1-1) from Atractylodes macrocephala with anti-weightlessness bone loss activity.

Carbohydr Polym. 2022 Oct 15;294:119742.


Rai I, Ahirwar A, Rai A, Varjani S, Vinayak V.

Biowaste recycling strategies for regenerative life support system: An overview.

Sustain Energy Technol Assess. 2022 Oct;53:102525.

Note: From the abstract: “Space missions are expanding their horizons towards green energy. At the same time, humans are expanding their invasion beyond the Earth’s orbit and envisage to have a habitat on other planets like Mars. This is possible only when there is a continuous efflux of human biowaste and influx of vital and breathable air, food and water for the habitants. The current scenario of space toilets is limited to recycling of human urine to drinkable water by refluxing the human liquid waste via Micro-Ecological Life Support System Alternative (MELiSSA) in a closed loop. On the offset, the human biowaste is thrown in space adding into space waste leading to another causes of concern for the space environment. To add up if human biowaste is recycled via photosynthetic microalgal microbial fuel cell integrated into MELiSSA project, it would be very useful since not only the microalgae will assimilate CO2

 exhaled by crew members but also give O2

 and edible biomass for their survival in the spacecraft. Simultaneously, this might reduce the payload during space voyage. The novelty of cultivating microalgae in spacecrafts for bioregenerative life support lies in further cultivating microalgae in Mars for its terraformation to planet Earth.”


Zhou Z, Xu B, Hu N, Guo Z, Bao W, Shao B, Yang W.

Targeting the macrophage-ferroptosis crosstalk: A novel insight into tumor immunotherapy.

Front Biosci (Landmark Ed). 2022 Jun 27;27(7):203. Review.

Note: This article may be obtained online without charge.


Mantua J, Shevchik JD, Chaudhury S, Eldringhoff HP, Mickelson CA, McKeon AB.

Sleep and infantry battle drill performance in special operations soldiers.

Aerosp Med Hum Perform. 2022 Jul 1;93(7):557-61.


McMullan CJ, McHill AW, Hull JT, Wang W, Forman JP, Klerman EB.

Sleep restriction and recurrent circadian disruption differentially affects blood pressure, sodium retention, and aldosterone secretion.

Front Physiol. 2022 July 8;13:914497.

Note: This article is part of Research Topic “Influence of Sleep and Recurrent Circadian Disruption on Cardiometabolic Health, Wellbeing, and Safety: From Shiftwork to Monday Mornings” ( This article may be obtained online without charge.


Smith MG, Cordoza M, Basner M.

Environmental noise and effects on sleep: An update to the WHO systematic review and meta-analysis.

Environ Health Perspect. 2022 Jul;130(7):76001. Review.

Note: This article may be obtained online without charge.


Hindorf M, Berggren P, Jonson CO, Lundberg L, Jonsson A.

Workload of Swedish Special Forces operators experienced during stressful simulation training: A pilot study.

J Spec Oper Med. 2022 Jul 25. Online ahead of print.

Note: NASA Task Load Index was used in this study. From the abstract: “Stress week was included during training of Special Forces (SF) Operators in Sweden to test their ability and limits for handling stress in different unknown situations and environments at a military training facility in Sweden. The aim of the study was to examine the effects of stress and workload experienced in various tasks during firefighting and military medicine simulation training.”


Ktistakis E, Skaramagkas V, Manousos D, Tachos NS, Tripoliti E, Fotiadis DI, Tsiknakis M.

COLET: A dataset for cognitive workload estimation based on eye-tracking.

Comput Methods Programs Biomed. 2022 Sep;224:106989.

Note: This article may be obtained online without charge.


Liu Z, Tang W, Liu J, Han Y, Yan Q, Dong Y, Liu X, Yang D, Ma G, Cao H.

A novel sprayable thermosensitive hydrogel coupled with zinc modified metformin promotes the healing of skin wound.

Bioact Mater. 2023 Feb;20:610-26.

Note: This article may be obtained online without charge.


Tajino J, Aoyama T, Kuroki H, Ito A.

Comprehensive understanding of inactivity-induced gait alteration in rodents.

J Vis Exp. 2022 Jul 6(185):e63865.

SpaceRef co-founder, Explorers Club Fellow, ex-NASA, Away Teams, Journalist, Space & Astrobiology, Lapsed climber.