NASA ISS On-Orbit Status 7 May 2014

Today: Threshold Acceleration for Gravisensing (GRAVI)-2 Run #2: Flight Engineer (FE)-5 Mastracchio hydrated sixteen GRAVI-2 sample chambers on the MWA.
He then assembled sixteen GRAVI-2 culture chambers and eight GRAVI-2 fixative units into the European Modular Cultivation System (EMCS) experiment containers, using the BioLab glove box. The aim of GRAVI-2 is to subject lentil seedling roots to different centrifugal acceleration levels in microgravity, in order to determine the threshold of acceleration the roots respond to. The seedling roots are stimulated by means of a centrifuge for several hours, and the gravitropic response (root curvature) is followed by time-lapse photography or video observation during this centrifugation; making it possible to determine precisely the threshold acceleration at which the root responds to the gravity stimulus. After the stimulation period, the roots are chemically fixed in glutaraldhyde, formaldehyde, and RNALater to determine the movement of amyloplasts under the influence of the stimulation.

Circadian Rhythms: ISS CDR Wakata completed his final 36 hour Circadian Rhythms session and removed the equipment. Circadian Rhythms investigates the role of synchronized circadian rhythms, or the “biological clock,” and how it changes during long-duration spaceflight. Researchers hypothesize that a non-24-hour cycle of light and dark affects crewmembers’ circadian clocks. The investigation also addresses the effects of reduced physical activity, microgravity and an artificially controlled environment. Changes in body composition and body temperature, which also occur in microgravity, can affect crewmembers’ circadian rhythms as well. Understanding how these phenomena affect the biological clock will improve performance and health for future crewmembers.

Biological Rhythms: Wakata attached a digital walk holter and electrodes to start taking data for his second measurement of Biological Rhythms. Wakata will collect data for 48 hours. Biological Rhythms 48 hours studies the effects of long-term microgravity exposure on heart function by analyzing an astronaut’s electrocardiogram for 48 hours.

Biological Research in Canisters (BRIC)-18 Canister D Activation: Mastracchio activated BRIC-18 Canister D. Later in the day, Mastracchio placed BRIC-18 Canister D into the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). The BRIC-18 investigation focuses on the growth and development of seedlings in microgravity. Seedlings will be preserved with a chemical fixative and returned to the ground for postflight evaluation.

Veggie Hardware Installation: FE-3 Swanson and Mastracchio installed the Veggie hardware into Express Rack (ER)-3. Swanson also completed Veggie software and VEG-01 experiment On-Board Training (OBT). Veggie provides the necessary lighting and nutrient delivery for efficient plant growth in space. The Veg-01 hardware validation test investigation utilizes the Veggie facility on ISS. This investigation will assess on-orbit function and performance of the Veggie, and focus on the growth and development of ‘Outredgeous’ Lettuce (Lactuca sativa ) seedlings in the spaceflight environment and the effects of the spaceflight environment on composition of microbial flora on the Veggie-grown plants and the Veggie facility.

Optical PAyload for Lasercomm Science (OPALS): Today Robotics Ground Controllers successfully installed OPALS on Express Logistics Carrier (ELC)1. Initial power on of the payload is planned for Saturday. OPALS tests the potential for using a laser to transmit data to Earth from space. Instead of being broadcast on radio waves, data is packaged onto beams of laser light.

In Flight Maintenance (IFM) Node 2 Common Berthing Mechanism (CBM) Bolt 3-1 Remove and Replace (R&R): On April 20 during SpX-3 berthing, CBM Bolt 3-1 failed its torque limit and was subsequently retracted. The bolt needed to be changed out prior to Dragon departure scheduled for May 18, 2014. Today Wakata and Swanson performed the bolt R&R and performed a video inspection of the bolt threads.

External (EXT)-2 Multiplexer/Demultiplexer (MDM) Card Removal – The crew removed the Enhanced Input Output Control Unit Circuit Card (EIOCC) and two power supply cards from the failed EXT-2 MDM. These cards will be returned to the ground for diagnostics.

Today’s Planned Tasks

SM ??? (Caution & Warning Panel) Test [Complete]
Laptop RS1(2) Reboot [Complete]
RSS1,2 Reboot / r/g 1009 [Complete]
HRF – Sample collection in preparation for cold stowage insertion. [Complete]
Reaction Self Test [Complete]
HRF – Sample MELFI Insertion [Complete]
GRAVI – Camcorder Setup [Complete]
Daily Planning Conference (S-band) [Complete]
GRAVI2 – Chamber Hydration for Culture experiment [Complete]
PETRI – Turning Lights On [Complete]
CRHYT – Deinstrumentation [Complete]
CONYUGATSIYA. Incubator Activation at +29 deg ? / r/g 5574 [Complete]
OBSTANOVKA. Download of Obstanovka P/L Data from ???? to RSS1 (start) / r/g 5161 [Complete]
Charging GoPro HERO3 Camcorder Battery – start / r/g 5570 [Complete]
URAGAN. Photo Spectrum System Battery Charge (start) / r/g 3620 [Complete]
Download Pille Dosimeter Readings / r/g 5564 [Complete]
?? 711 Transfer Ops / r/g 5526 [Complete]
CRHYT – Data Downlink [Complete]
VEGGIE – Equipment Setup [Complete]
Installation and Connection of EKTS Antenna Feeder #540?=WS2 switch. / r/g 5563 [Complete]
GRAVI2 – H/W assembly for experiment run 2 [Complete]
VEGGIE – Training Exercise [Complete]
Replace Pille Flash Card/ r/g 5564 [Complete]
Mating EKTS Antenna Feeder #540?=WS2 switch TLM connectors. / r/g 5563 [Complete]
Installing and Connecting EKTS Antenna Feeder Cables (17??.540?8254?-20, -50, – 60). / r/g 5563 [Complete]
CONYUGATSIYA. Removal of Recomb-K from thermostat (+4 deg C) and Insertion into thermostat at +29 deg C. / r/g 5574 [Complete]
ISS Crew / SSIPC FD Conference [Complete]
??-1 Flow Sensor Position Check [Complete]
Marangoni Deformation 10 (MD10) Resistance Measurement [Complete]
CONYUGATSIYA. Process activation, setup in ???-? at +29 deg. / r/g 5574 [Complete]
CONYUGATSIYA. Photography during the activation / r/g 5580 [Complete]
Verification that Node 3 camcorder is powered on to ensure exercise video is downlinked [Complete]
EMCS – Container Assembly installation [Complete]
IDENTIFIKATSIYA. Copy ???-? micro-accelerometer data to laptop / r/g 5186 [Complete]
In-Flight Maintenance (IFM) – Bolt R&R [Complete]
OBSTANOVKA. Download of Obstanovka P/L Data from ???? to RSS1 (end) / r/g 5161 [Complete]
Physical Fitness Evaluation (on treadmill). / r/g 5572 [Aborted]
Charging GoPro HERO3 Camcorder Battery – end / r/g 5570
WRM Condensate Pumping Start [Complete]
Verification of settings and clearing GoPro HERO3 flash-cards prior to ?? 711 Descent Ops. Removal of attachment plate SM-GoPro-U08 in ?? 711 ?? for descent stowage. / r/g 5570
CMRS checkout [Complete]
URAGAN. Observation and Photography using Photo Spectrum System / r/g 5575 [Complete]
Consultation on GoPro HERO3 Camcorder Operation and Setup in ?? 711 [??]. / r/g 5570
CONYUGATSIYA. Complete activation process, set up in thermostat (+29 deg C). / r/g 5574 [Complete]
WRM Condensate Pumping Terminate [Complete]
BLR48 – starting measurement [Complete]
37S Transfer Ops / r/g 5526
Camcorder Stowage [Complete]
EHS TOCA waste water bag change out [Complete]
BRIC – Activation [Complete]
BRIC. Historical Documentation [Complete]
WRS Water Sample Analysis [Complete]
Audio Journal Entry FE3 [Complete]
CONYUGATSIYA. Retrieval from thermostat (+29 deg ?) and transfer to thermostat (+4 deg ?) / r/g 5574 [Complete]
VEGGIE – Hardware On-Board Training [Complete]
??? Maintenance
CONYUGATSIYA. Thermostat Removal / r/g 5574 [Complete]
OBT- Dragon Robot setup [Complete]
In-Flight Maintenance (IFM) – Bolt R&R [Complete]
Journal Entry [Complete]
Changeout of ????-2? purification column assembly [???] / r/g 5576 [Complete]
ALBEDO. Photo Spectral System (???) Prep, Imagery, Closeout Ops / r/g 5571 [Aborted]
37S Transfers Complete Report / r/g 5526
Crew Departure Prep
NANO – BioRack Experiment Containers and Controllers Removal [Complete]
Physical Fitness Evaluation (on treadmill). / r/g 5572
IMS Update
BIORAK – Glacier Container Insertion [Complete]
VEGGIE – Equipment Installation and Connection [Complete]
CIRCADIAN RHYTHMS – Remove and Trash Old Batteries [Complete]
VEGGIE – Software On-Board Training [Complete]
Recording of Video Greetings / r/g 5578
BRIC – Sample Insertion into MELFI [Complete]
VEG – Training Exercise [Complete]
TOCA Data Recording [Complete]
Daily Planning Conference (S-band) [Complete]
PETRI – Turning lights off [Complete]
Reaction Self Test [Complete]
Task List

ARED Cylinder Evac
WHC KTO Replace
WHC EDV R&R
Roscosmos Report
Uragan Photo
Ekon Photo
37S US prepack [In work]
XFER Dragon Cargo Ops [In work]
SSC12 recovery [Complete]
Crew departure prep (CDR) [In work]
Data prep return (FE-5) [In work]
FPS tool gather [In work]
NOD1OS2 LHA R&R
NOD1PD2-LHA R&R
ST ISS Read
MDM card removal [Complete]
MOOT restow
Ground

OPALS installation onto ELC1 [Complete]
Nominal commanding
Three-Day Look Ahead:

Thursday, 05/08: CsPINs, OBT Soyuz drill 2, Petri Plate ops, Veg-01 experiment start, crew departure prep, SMILES relocation
Friday, 05/09: EMCS Exp. Container insertion, ScPINs Sample Fixation 1,2, Dragon Cargo ops, OBT Dragon RoOBT, NanoRacks Module-27 swab ops, GRAVI2, MWA equipment deconfigure, 37S pack/stow, crew departure prep, STPH4 and SCAN Testbed survey
Saturday, 05/10: Crew off duty, APEX Plate swap
QUICK ISS Status – Environmental Control Group:

Component – Status
Elektron – On
Vozdukh – Manual
[???] 1 – SM Air Conditioner System (“SKV1”) – On
[???] 2 – SM Air Conditioner System (“SKV2”) – Off
Carbon Dioxide Removal Assembly (CDRA) Lab – Standby
Carbon Dioxide Removal Assembly (CDRA) Node 3 – Operate
Major Constituent Analyzer (MCA) Lab – Stop
Major Constituent Analyzer (MCA) Node 3 – Operate
Oxygen Generation Assembly (OGA) – Process
Urine Processing Assembly (UPA) – Standby
Trace Contaminant Control System (TCCS) Lab – Off
Trace Contaminant Control System (TCCS) Node 3 – Full up