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NASA Spaceline Current Awareness List #1,024  11 November 2022 (Space Life Science Research Results)

By Keith Cowing
Press Release
November 11, 2022
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NASA Spaceline Current Awareness List #1,024  11 November 2022 (Space Life Science Research Results)
Space Biology

SPACELINE Current Awareness Lists are distributed via listserv and are available on the NASA Task Book website at Please send any correspondence to Shawna Byrd, SPACELINE Current Awareness Senior Editor,

Call for articles to cite in the weekly lists: Authors at NASA Centers and NASA PIs—do you have an article that has recently published or will publish in the upcoming weeks within a peer-reviewed journal and is in the scope of space life sciences? If so, send it our way! Send your article to the email address mentioned above. Articles received by Wednesday will appear within that week’s list—articles received after Wednesday will appear the following week.

Papers deriving from NASA support:


Soni P, Anupom T, Lesanpezeshki L, Rahman M, Hewitt JE, Vellone M, Stodieck L, Blawzdziewicz J, Szewczyk NJ, Vanapalli SA.

Microfluidics-integrated spaceflight hardware for measuring muscle strength of Caenorhabditis elegans on the International Space Station.

npj Microgravity. 2022 Nov 7;8:50.

PI: S.A. Vanapalli

Note: ISS results.

Journal Impact Factor: 4.97

Funding: “This work was supported by funding from NASA (Grant No. NNX15AL16G and NNJ15GK01C) and BBSRC (Grant No. BB/N015894/1).”


Overbey EG, Das S, Cope H, Madrigal P, Andrusivova Z, Frapard S, Klotz R, Bezdan D, Gupta A, Scott RT, Park J, Chirko D, Galazka JM, Costes SV, Mason CE, Herranz R, Szewczyk NJ, Borg J, Giacomello S.

Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.

Cell Rep. 2022 Oct 31;100325. Online ahead of print.

PI: E.G. Overbey, NASA Postdoctoral Fellowship

Note: From the introduction: “For decades, the health risks associated with space exploration have been increasingly documented and characterized. For some of these risks, countermeasures have been sufficiently developed to sustain human health in low-Earth orbit, the orbital distance from Earth that includes the International Space Station (ISS). However, as humankind plans for a permanent presence on the moon and seeks to advance to Mars, the limits to our countermeasures and the research gaps that have insofar remained unaddressed will create health challenges that rival the engineering challenges of long-duration space missions.”

Journal Impact Factor: 9.995

Funding: “H.C., P.M., D.B., R.H., N.J.S., J.B., and S.G. are members of the ESA Space Omics Topical Team, funded by the ESA grant/contract 4000131202/20/NL/PG/pt ‘Space Omics: Towards an integrated ESA/NASA–omics database for spaceflight and ground facilities experiments’ awarded to R.H., which was the main funding source for this work. H.C. is also supported by the Horizon Centre for Doctoral Training at the University of Nottingham (UKRI grant no. EP/S023305/1). S.G. is supported by the Swedish Research Council VR grant 2020-04864. E.G.O. is supported through NASA Postdoctoral Fellowship 80NSSC21K0316.”


Siems K, Müller DW, Maertens L, Ahmed A, Van Houdt R, Mancinelli RL, Baur S, Brix K, Kautenburger R, Caplin N, Krause J, Demets R, Vukich M, Tortora A, Roesch C, Holland G, Laue M, Mücklich F, Moeller R.

Testing laser-structured antimicrobial surfaces under space conditions: The design of the ISS experiment BIOFILMS.

Front Space Technol. 2022 Jan 3;2:773244.

PI: R.L. Mancinelli

Note: From the abstract: “The bacterial cells of the three species included in BIOFILMS need to be stored in the flight hardware as pre-test inoculation cultures until the experiment is activated aboard the ISS. For this, we considered liquid buffers that fulfilled specific requirements. Namely, the storage buffer needs to limit bacterial activity during the storage phase, but keep the cells in an intact and viable condition allowing growth when the experiment is activated. Furthermore, in the selection process for the liquid growth medium that will be present during the incubation and biofilm formation phase of the experiment various factors were considered. Since different bacterial species need different amounts of nutrient for growth and biofilm formation, the growth medium will need to support growth and biofilm formation of all three selected strains.” This article may be obtained online without charge.

Journal Impact Factor: Not available for this journal

Funding: “KS and RM were supported by the DLR grant FuE-Projekt ‘ISS LIFE’ (Programm RF-FuW, Teilprogramm 475). DM and AA were supported by German Aerospace Center—Space Administration (DLR) within the project ‘Investigation of antimicrobial metal surfaces under space conditions—An effective strategy to prevent microbial biofilm formation’ (project number 50WB1930). LM and RH were supported by the European Space Agency (ESA-PRODEX) and the Belgian Science Policy (Belspo) through the BIOFILMS project (C4000129318). RLM was supported by NASA Space Biology grant 80NSSC18K0751 (BIOFILMS) to the Bay Area Environmental Research Institute.”


Waisberg E, Ong J, Paladugu P, Kamran SA, Zaman N, Lee AG, Tavakkoli A.

Challenges of artificial intelligence in space medicine.

Space Sci & Technol. 2022 Oct 29;2022:9852872.

PI: A. Tavakkoli

Note: This article may be obtained online without charge.

Journal Impact Factor: Not available for this journal

Funding: “This study was supported by NASA Grant [80NSSC20K183]: A Non-intrusive Ocular Monitoring Framework to Model Ocular Structure and Functional Changes due to Long-term Spaceflight.”


Waisberg E, Ong J, Paladugu P, Zaman N, Kamran SA, Lee AG, Tavakkoli A.

Optimizing screening for preventable blindness with head-mounted visual assessment technology.

J Vis Impair Blind. 2022 Jul 1;116(4):579-81.

PI: A. Tavakkoli

Journal Impact Factor: 1.128

Funding: “The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: NASA Grant No. 80NSSC20K183: A non-intrusive ocular monitoring framework to model ocular structure and functional changes due to long-term spaceflight.”


Sater SH, Conley Natividad G, Seiner AJ, Fu AQ, Shrestha D, Bershad EM, Marshall-Goebel K, Laurie SS, Macias BR, Martin BA.

MRI-based quantification of posterior ocular globe flattening during 60 days of strict 6° head-down tilt bed rest with and without daily centrifugation.

J Appl Physiol (1985). 2022 Nov 3.

PIs: E.M. Bershad, B.A. Martin

Note: Head-down tilt bedrest study.

Journal Impact Factor: 3.880

Funding: PIs report NASA funded SPACE-CENT study and OPTICS project funding.


Duty L, Paul AM.

Isolation of murine bone marrow-derived neutrophils for infection modeling.

In: Bai F, ed. West Nile Virus. Methods Mol Biol.

Vol. 2585. New York: Humana, 2022. p. 33-40.

Funding: A. Paul is affiliated with NASA Ames Research Center.


Hall MK, Thiel J, Dunmire B, Samson PC, Kessler R, Sunaryo P, Sweet RM, Metzler IS, Chang HC, Gunn M, Dighe M, Anderson L, Popchoi C, Managuli R, Cunitz BW, Burke BH, Ding L, Gutierrez B, Liu Z, Sorensen MD, Wessells H, Bailey MR, Harper JD.

First series using ultrasonic propulsion and burst wave lithotripsy to treat ureteral stones.

J Urol. 2022 Nov 1;208(5):1075-82.

PIs: M.K. Hall, H. Wessells, M.R. Bailey

Note: This article may be obtained online without charge.

Journal Impact Factor: 7.600

Funding: “The study was supported by NASA HRP ExMC MTL ID 1265 through a contract with ZIN Technologies. System and research infrastructure development was supported by NIH NIDDK P01 DK043881, NIH K01 DK104854, and the National Space Biomedical Research Institute.”


Other papers of interest:


Arbeille P, Greaves D, Guillon L, Hughson RL.

4 days in dry immersion increases arterial wall response to ultrasound wave as measured using radio-frequency signal, comparison with spaceflight data.

Front Physiol. 2022 Nov 8;13:983837.

Note: ISS results. This article may be obtained online without charge.


Rybalchenko OV, Orlova OG, Kapustina VV, Popova EV, Kutnik IV.

Trends in formation of microbial communities formed by probiotic bacteria Lactobacillus plantarum 8pa-3 on various carriers in the space flight environment.

Aviakosm Ekolog Med. 2022;56(5):85-95. Russian.

Note: ISS results. From the abstract: “This paper presents comparative analysis of microbial communities formed by probiotic lactobacteria Lactobacillus plantarum8PA-3 on various carriers (space experiment Biofilm) during ISS missions 53, 54 and 56.”


Jacob P, Bonnefoy J, Ghislin S, Frippiat JP.

Long-duration head-down tilt bed rest confirms the relevance of the neutrophil to lymphocyte ratio and suggests coupling it with the platelet to lymphocyte ratio to monitor the immune health of astronauts.

Front Immunol. 2022 Oct 13;13:952928.

Note: Head-down tilt bedrest study. This article is part of Research Topic “Deciphering Immune and Neuroendocrine Crosstalk in Extreme Environments” ( The Research Topic also includes articles from previous Current Awareness Lists #965, #990, and #1,023 This article may be obtained online without charge.


D’Angelo O, Horb A, Cowley A, Sperl M, Kranz WT.

Granular piston-probing in microgravity: Powder compression, from densification to jamming.

npj Microgravity. 2022 Nov 5;8:48.

Note: From the introduction: “The aim of this article is to investigate the densification of a granular packing in two scenarios: on-ground (gnd), where Earth’s gravity may accelerate particles relative to the fixed container, and in microgravity (μ

-g), on parabolic flights, where gravity cannot induce such relative acceleration.”


Li Y, Kong C, Wang B, Sun W, Chen X, Zhu W, Ding J, Lu S.

Identification of differentially expressed genes in mouse paraspinal muscle in response to microgravity.

Front Endocrinol (Lausanne). 2022 Oct 13;13:1020743.

Note: From the abstract: “Lower back pain (LBP) is the primary reason leading to dyskinesia in patients, which can be experienced by people of all ages. Increasing evidence have revealed that paraspinal muscle (PSM) degeneration (PSMD) is a causative contributor to LBP. Current research revealed that fatty infiltration, tissue fibrosis, and muscle atrophy are the characteristic pathological alterations of PSMD, and muscle atrophy is associated with abnormally elevated oxidative stress, reactive oxygen species (ROS) and inflammation. Interestingly, microgravity can induce PSMD and LBP. However, studies on the molecular mechanism of microgravity in the induction of PSMD are strongly limited. This study identified 23 differentially expressed genes (DEGs) in the PSM (longissimus dorsi) of mice which were flown aboard the Bion M1 biosatellite in microgravity by bioinformatics analysis.” This article is part of Research Topic “Underlying Mechanisms and Treatment for Intervertebral Disc Disease” ( Additional articles will be forthcoming and may be found in the link to the Research Topic. This article may be obtained online without charge.


Luchitskaya ES, Funtova II.

Possibilities to study force and energy characteristics of the cardiac muscle in microgravity using ballistocardiography.

Aviakosm Ekolog Med. 2022;56(5):60-4. Russian.


Mochi F, Scatena E, Rodriguez D, Ginebra MP, Del Gaudio C.

Scaffold-based bone tissue engineering in microgravity: Potential, concerns and implications.

npj Microgravity. 2022 Oct 29;8:45. Review.

Note: From the abstract: “One of humanity’s greatest challenges is space exploration, which requires an in-depth analysis of the data continuously collected as a necessary input to fill technological gaps and move forward in several research sectors. Focusing on space crew healthcare, a critical issue to be addressed is tissue regeneration in extreme conditions. In general, it represents one of the hottest and most compelling goals of the scientific community and the development of suitable therapeutic strategies for the space environment is an urgent need for the safe planning of future long-term crewed space missions. Osteopenia is a commonly diagnosed disease in astronauts due to the physiological adaptation to altered gravity conditions. In order to find specific solutions to bone damage in a reduced gravity environment, bone tissue engineering is gaining a growing interest. With the aim to critically investigate this topic, the review presented here reports and discusses bone tissue engineering scenarios in microgravity, from scaffolding to bioreactors.”


Barantseva MY, Mukhamedieva LN, Ozerov DS, Pakhomova AA, Lashukov PV, Grabeklis IA.

Toxicology issues of lunar dust effects on humans.

Aviakosm Ekolog Med. 2022;56(5):29-37. Review. Russian.

Note: From the abstract: “Long-term lunar missions will require a toxicological and hygienic assessment of the biological impact of lunar dust on humans, as one of the main factors limiting the duration of human stay on the lunar surface. This review summarizes the results of scientific research aimed at studying the acute and chronic effects of lunar dust and its imitators when inhaled.”


Allen BD, Alaghband Y, Kramár EA, Ru N, Petit B, Grilj V, Petronek MS, Pulliam CF, Kim RY, Doan NL, Baulch JE, Wood MA, Bailat C, Spitz DR, Vozenin MC, Limoli CL.

Elucidating the neurological mechanism of the FLASH effect in juvenile mice exposed to hypofractionated radiotherapy.

Neuro Oncol. 2022 Nov 5. Online ahead of print.

10Belova SP, Zaripova KА, Sharlo KА, Shenkman BS, Nemirovskaya TL.

Role of AMP-activated protein kinase in the regulation of calcium-dependent and ubiquitin-proteasome signaling pathways during muscle unloading.

Aviakosm Ekolog Med. 2022;56(5):65-76. Russian.

Note: Hindlimb unloading study.


Berkovich YA, Shalopanova OA, Buryak AA.

Optimization of the plant illumination system stabilizing oxygen concentration in the gaseous environment within a prototyped biotechnical crew life support system.

Aviakosm Ekolog Med. 2022;56(5):102-10. Russian.


Nakdimon I, Ben-Ari O.

Mitigating risks of altitude chamber training.

Aerosp Med Hum Perform. 2022 Nov 1;93(11):811-5.

Note: From the abstract: “Altitude chambers are used for training aircrews in a hypobaric hypoxic environment to better prepare them for pressurization and oxygen malfunction incidents during flights. However, adverse effects may occur during training sessions, with decompression sickness (DCS) being a major concern. The aim of this study was to examine the risks of different adverse effects during altitude chamber trainings (ACT) in the Israeli Air Force (IAF) facility and to compare them to other training facilities.”


Bubeev YA, Usov VM, Polyakov AV.

Virtual reality-based modeling of the modes of piloted vehicle control on a lunar station to study operator activity in isolation studies.

Aviakosm Ekolog Med. 2022;56(5):14-28. Russian.

Note: From the abstract: “This review considers the possibility of using virtual reality technologies and 3D visualization to study in laboratory conditions the interaction of astronauts with robots in intra-ship activity (VnuKD).”


Gaynullina DK, Shvetsova AA, Tarasova OS.

Mechanisms of the acidosis effect on the vascular tone.

Aviakosm Ekolog Med. 2022;56(5):38-45. Russian.


Iordanishvili AK.

Parafunction of the muscles of mastication in flight personnel: Frequency of occurrence and pathogenesis (clinical experimental investigation).

Aviakosm Ekolog Med. 2022;56(5):96-101. Russian.


Savin SN, Voitulevich LV, Grishin AP, Kireev KS.

Analysis of the results of medical selection of cosmonaut candidates, 2019–2020.

Aviakosm Ekolog Med. 2022;56(5):46-50. Russian.

17Skedina МА, Kovaleva АА, Potapov MG.

The possibility of applying an integrated method of rapid central nervous system functioning assessment to cosmonauts.

Aviakosm Ekolog Med. 2022;56(5):51-9. Russian.


Sukhoterin AF, Paschenko PS, Shapovalov PA.

Structural transformations in the dorsal vagal complex after acute and chronic exposure to gravitational overload.

Aviakosm Ekolog Med. 2022;56(5):77-84. Russian.


Teaford M, Keller K, Merfeld DM.

The contribution of interoceptive signals to spatial orientation: A mini-review.

Neurosci Biobehav Rev. 2022 Dec;104943. Review.

Note: From the abstract: “Humans’ sensory systems work synergistically to allow us to determine where our head and body are relative to the environment. To date, most research on this topic has focused on the visual and vestibular systems. There has been much less research on the contributions of interoceptive signals to spatial orientation, so the overall picture of how spatial orientation works is incomplete. This gap in the literature is especially problematic for aviation and spaceflight, where spatial disorientation is more likely to occur due to less common gravity conditions (i.e. microgravity and hypergravity) and can lead to fatal errors. In the present manuscript we review and summarize the current literature on this topic.”

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